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訪客人次:564617
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科技部新聞稿 : 暢快呼吸-氣管移植新契機

以人工氣管進行氣管移植重建手術至今仍然是全球胸腔醫學最困難的挑戰。近年伴隨著結合幹細胞之組織工程技術的進展,以及3D列印等工程科技的進步,與人體氣管構造相近又具生理功能的仿生氣管成為解決此難題的契機。在科技部再生醫學科技計畫的支持下,台大陳晉興教授研究團隊結合六個各具自動化/力學/材料/幹細胞/醫學/倫理專長,且有高度互補性的教授與醫師團隊,一同為製作仿生氣管的理想而努力。

研究團隊從需要氣管移植的病人身上取下少量細胞,轉化為誘導性多功能幹細胞(induced pluripotent stem cell, iPSC)。體外大量培養這些幹細胞後,使之附著到客製化的3D列印氣管支架上。最後誘導這些幹細胞分化成為氣管相關細胞,形成可供移植的仿生氣管。

在體外大量培養幹細胞之步驟中,為減輕人力負擔及減少人因的病菌感染可能,以保障臨床使用安全,設計了利用自動化機械手臂來執行日常培養工作。同時,研發類神經網路程式來判斷細胞培養的狀況,及時回饋機械手臂來調整培養條件,除自動化外還達到智慧化細胞培養。

  另為刺激幹細胞增生與促進分化,研發超音波刺激技術與設備。藉由團隊組建的超音波設備,將機械波傳遞進入細胞,而激活細胞內部的生理反應。該團隊是學術上首次利用即時影像證實超音波可活化細胞的團隊。

  繼培養足量的高品質幹細胞後,利用團隊所研發之適合活體移植可生物降解的材料,做成氣管支架,以種植這些幹細胞。此支架具多功能:具專利的細節構造除允許幹細胞生長於其上之外,也能含入小分子藥物,可提供最有效的組織再生。另,支架係以3D列印的方式製造,其長短粗細等,可搭配醫學影像而達到客製化,符合個別病人的需求。

  目前臨床前技術與設備的重大研發成果,已取得專利及技術移轉。而動物實驗也正積極進行當中,後續更即將進入人體臨床驗證。

 

Free to breathe:

A new turning point of tracheal transplant

Tracheal reconstruction using artificial trachea is still a great challenge in thoracic medicine. With the advances of tissue engineering technology, stem cell biology, and 3D printing technology, the engineered bionic trachea that mimics tracheal anatomy and functions like human trachea have the potential to overcome the clinical barrier and regenerate tracheas. It begins with taking few cells from the patient needing tracheal reconstruction, and then transforming these cells into induced pluripotent stem cell. After in vitro cell expansion culture, the obtained stem cells will be transferred to a custom- designed 3D-prineted stent. Eventually, the stem cells on the stent will be induced and differentiated to mature trachea-related cells, becoming the bionic trachea ready for transplantation. Under the supports from Taiwan MOST, we recruited professors and doctors who major in automated intelligence, biomechanics, materials science, stem cell biology, medicine, and clinical ethics and work together to make the dream of bionic trachea come true. 

  For in vitro cell expansion, we designed automated robotic arm to perform daily works in cell culture. There are two advantages: 1) to reduce the workload by human, allowing researchers to do real researches but boring repetitions; 2) to avoid bacterial pollution from human, ensuring the safety in clinical applications. We also developed Artificial Neural Network for cell culture quality control. The obtained data will be fed back to robotic arm to responsively adjust the culture conditions. Therefore, our robotic arm is not only automated but also intellectualized.

  For promotion of stem cell proliferation/differentiation, we developed techniques and equipments based on ultrasound (US). US can deliver mechanical waves into cells, inducing responses inside the cells. By using our US equipments, we are the first one to visualize the US-induced cell activation. We saw lamellipodial, a typical response for cell activation, 10 minutes after US application.

  After preparation of stem cells with high quality, we then need stent to enclose these stem cells. Therefore, we developed bio-degradable stent material, which is suitable for human transplantation. This stent is multi- functions: its fine structure with patent allows the growth of stem cells and enclosure of small molecule drugs, thereby promoting regeneration of tracheal tissue. Moreover, out stent is 3D-printed, which is also custome- made when basing on medical imaging for individual patient.

  The aforementioned preclinical techniques and equipments has patent and technological transfer. In addition, animal study has been proceed and clinical study will be started soon as the proof of concept of engineered bionic trachea.

研究成果聯絡人

陳晉興醫師 Jin-Shing Chen

台大外科教授暨胸腔外科主任

Department of Surgery, National Taiwan University and National Taiwan University Hospital, Taipei

聯絡電話:23123456 #65178

電子郵件信箱:chenjs@ntu.edu.tw

更新日期 : 2018/12/20